Speedy protein A Antibody / SPDYA / Spy1
SPDYA (speedy homolog A (Drosophila) ) regulates the G1/S phase transition of the cell cycle by binding and activating CDC2, CDK2 and CDKN1B/KIP1. SPDYA can activate CDK2 without promoting CDK2 phosphorylation. SPDYA mediates cell survival during the DNA damage process through activation of CDK2.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100
UniProt
Q5MJ70
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 147-175 from the human protein was used as the immunogen for the Speedy protein A antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the Speedy protein A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Speedy protein A antibody is available for research use only.
Storage Conditions
Aliquot the Speedy protein A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Predominantly nuclear, may show cytoplasmic staining in some tissue
Image Legend
Western blot testing of 1) non-transfected and 2) transfected 293 cell lysate with Speedy protein A antibody. Predicted molecular weight ~36 kDa.
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