CaI-PLA2 Antibody / PLA2G6

The protein encoded by this gene is an A2 phospholipase, a class of enzyme that catalyzes the release of fatty acids from phospholipids. The encoded protein may play a role in phospholipid remodelling, arachidonic acid release, leukotriene and prostaglandin synthesis, fas-mediated apoptosis, and transmembrane ion flux in glucose-stimulated B-cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

O60733

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 551-580 from the human protein was used as the immunogen for the CaI-PLA2 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PLA2G6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CaI-PLA2 antibody is available for research use only.

Storage Conditions

Aliquot the PLA2G6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CaI-PLA2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human testis carcinoma tissue with CaI-PLA2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.