Mitoferrin-1 Antibody / SLC25A37 / MFRN

Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe2+, is then probably delivered to ferrochelatase to catalyze Fe2+ incorporation into protoprophyrin IX to make heme (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50

UniProt

Q9NYZ2

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 309-338 from the human protein was used as the immunogen for the Mitoferrin-1 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Mitoferrin-1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Mitoferrin-1 antibody is available for research use only.

Storage Conditions

Aliquot the Mitoferrin-1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Mitoferrin-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human brain tissue with Mitoferrin-1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.