Beta Glucuronidase Antibody / Gusb

The GUSB gene encodes beta-glucuronidase, a lysosomal hydrolase involved in the stepwise degradation of glucuronic acid-containing glycosaminoglycans (Shipley et al., 1993) . It is a tetrameric glycoprotein composed of identical subunits (Oshima et al., 1987) . The GUSB gene is mutated in mucopolysaccharidosis type VII.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:2000-1:4000

UniProt

P08236

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 335-362 from the human protein was used as the immunogen for the Beta Glucuronidase antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, FACS, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Beta Glucuronidase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Beta Glucuronidase antibody is available for research use only.

Storage Conditions

Aliquot the Beta Glucuronidase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Beta Glucuronidase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human liver tissue with Beta Glucuronidase antibody. HIER: steam section in pH9 EDTA buffer for 20 min and allow to cool prior to staining.