Topoisomerase II alpha Antibody / TOP2A

TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P11388

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1167-1196 from the human protein was used as the immunogen for the Topoisomerase II alpha antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Topoisomerase II alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Topoisomerase II alpha antibody is available for research use only.

Storage Conditions

Aliquot the Topoisomerase II alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Topoisomerase II alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear, cytoplasmic

Image Legend

IHC testing of FFPE human skin tissue with Topoisomerase II alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.