Ataxin 3 Antibody / ATXN3

ATXN3 was known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein contains (CAG) n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P54252

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 261-288 from the human protein was used as the immunogen for the Ataxin 3 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Ataxin 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Ataxin 3 antibody is available for research use only.

Storage Conditions

Aliquot the Ataxin 3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Ataxin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE human brain tissue with Ataxin 3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.