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RPL18A Antibody / 60S ribosomal protein L18a / Large ribosomal subunit protein eL20

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL18A is a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L18AE family of ribosomal proteins. It is located in the cytoplasm.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q02543

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 140-166 from the human protein was used as the immunogen for the RPL18A antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RPL18A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RPL18A antibody is available for research use only.

Storage Conditions

Aliquot the RPL18A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the RPL18A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human HEK293 cell lysate with RPL18A antibody. Predicted molecular weight ~21 kDa.

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