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POU2AF1 Antibody / BOB-1

POU2AF1 is transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:1000-1:2000

UniProt

Q16633

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-27 from the human protein was used as the immunogen for the POU2AF1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IHC-P, FACS, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the POU2AF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This POU2AF1 antibody is available for research use only.

Storage Conditions

Aliquot the POU2AF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the POU2AF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

Flow cytometry testing of human Ramos cells with POU2AF1 antibody; Blue=isotype control, Green= POU2AF1 antibody.

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