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EFEMP1 Antibody / Fibulin 3

EFEMP1 encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, EGF-containing fibulin-like extracellular matrix protein 1 contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q12805

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 119-148 from the human protein was used as the immunogen for the EFEMP1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the EFEMP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This EFEMP1 antibody is available for research use only.

Storage Conditions

Aliquot the EFEMP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the EFEMP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) A431 and 2) HUVEC lysate with EFEMP1 antibody. Predicted molecular weight ~54 kDa.

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