Clathrin Light Chain A Antibody / CTLA

Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P09496

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 99-127 from the human protein was used as the immunogen for the Clathrin Light Chain A antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Clathrin Light Chain A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Clathrin Light Chain A antibody is available for research use only.

Storage Conditions

Aliquot the Clathrin Light Chain A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Clathrin Light Chain A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human lung carcinoma tissue with Clathrin Light Chain A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.