APEX2 Antibody

APEX2 may participate in both nuclear and mitochondrial post-replicative base excision repair (BER) . In the nucleus functions in the PCNA-dependent BER pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

Q9UBZ4

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 143-171 from the human protein was used as the immunogen for the APEX2 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the APEX2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This APEX2 antibody is available for research use only.

Storage Conditions

Aliquot the APEX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the APEX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human lung carcinoma tissue with APEX2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.