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Cdc37 Antibody

Cdc37 is a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:1000-1:2000

UniProt

Q16543

Host

Rabbit

Reactivity

Human, Rat

Immunogen

A portion of amino acids 116-144 from the human protein was used as the immunogen for the Cdc37 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Cdc37 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Cdc37 antibody is available for research use only.

Storage Conditions

Aliquot the Cdc37 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Cdc37 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human A431, 2) rat C6, 3) human Jurkat, 4) human K562 and 5) human LNCaP cell lysate with Cdc37 antibody. Expected molecular weight: 44-50 kDa.

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