GPA33 Antibody / Cell surface A33 antigen

GPA33 is a cell surface antigen that is expressed in greater than 95% of human colon cancers. It may play a role in cell-cell recognition and signaling.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q99795

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 264-293 from the human protein was used as the immunogen for the GPA33 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GPA33 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GPA33 antibody is available for research use only.

Storage Conditions

Aliquot the GPA33 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GPA33 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

Western blot testing of human 1) 293T and 2) HT-29 cell lysate with GPA33 antibody. Expected molecular weight: 33-43 kDa depending on glycosylation level.