Galactosylceramide sulfotransferase Antibody / GAL3ST1

Sulfonation, an important step in the metabolism of many drugs, xenobiotics, hormones, and neurotransmitters, is catalyzed by sulfotransferases. GAL3ST1 is galactosylceramide sulfotransferase which catalyzes the conversion between 3'-phosphoadenylylsulfate + a galactosylceramide to adenosine 3',5'-bisphosphate + galactosylceramide sulfate. Activity of this sulfotransferase is enhanced in renal cell carcinoma.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:2000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q99999

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 88-116 from the human protein was used as the immunogen for the Galactosylceramide sulfotransferase antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Galactosylceramide sulfotransferase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Galactosylceramide sulfotransferase antibody is available for research use only.

Storage Conditions

Aliquot the Galactosylceramide sulfotransferase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Galactosylceramide sulfotransferase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of 1) human kidney and 2) mouse kidney tissue lysate with Galactosylceramide sulfotransferase antibody. Predicted molecular weight ~49 kDa.