COTL1 Antibody / Coactosin-like protein

COTL1 is one of the numerous actin-binding proteins which regulate the actin cytoskeleton. This protein binds F-actin, and also interacts with 5-lipoxygenase, which is the first committed enzyme in leukotriene biosynthesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q14019

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 115-142 from the human protein was used as the immunogen for the COTL1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the COTL1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This COTL1 antibody is available for research use only.

Storage Conditions

Aliquot the COTL1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the COTL1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human spleen tissue with COTL1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.