CYP7B1 Antibody

CYP7B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)

UniProt

O75881

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 252-281 from the human protein was used as the immunogen for the CYP7B1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC-P, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the CYP7B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CYP7B1 antibody is available for research use only.

Storage Conditions

Aliquot the CYP7B1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the CYP7B1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.