SREBF1 Antibody / Sterol regulatory element-binding protein 1
SREBF1 is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000
UniProt
P36956
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 349-378 from the human protein was used as the immunogen for the SREBF1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
FACS, IHC-P, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the SREBF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This SREBF1 antibody is available for research use only.
Storage Conditions
Aliquot the SREBF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of human MDA-MB-435 cell lysate with SREBF1 antibody. Predicted molecular weight ~122 kDa.
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