PHYH Antibody / Phytanoyl-CoA dioxygenase, peroxisomal

PHYH2 catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

O14832

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 59-87 from the human protein was used as the immunogen for the PHYH antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PHYH antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PHYH antibody is available for research use only.

Storage Conditions

Aliquot the PHYH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PHYH antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skeletal muscle tissue with PHYH antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.