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PTK2 Antibody / FAK / Focal Adhesion Kinase

PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q05397

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 821-848 from the human protein was used as the immunogen for the PTK2 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PTK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PTK2 antibody is available for research use only.

Storage Conditions

Aliquot the PTK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PTK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear, extracellular

Image Legend

IHC testing of FFPE human brain tissue with PTK2 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

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