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PKC alpha Antibody / PRKCA

PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:25, Flow cytometry: 1:25 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:25, Western blot: 1:500-1:2000

UniProt

P17252

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 75-103 from the human protein was used as the immunogen for the PKC alpha antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PKC alpha antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PKC alpha antibody is available for research use only.

Storage Conditions

Aliquot the PKC alpha antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.nsjbio.com/tds-pdf/pkc-alpha-antibody-prkca-f54980

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human lung carcinoma tissue with PKC alpha antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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