PSMB9 Antibody / Proteasome subunit beta type-9

PSMB9 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P28065

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 193-219 from the human protein was used as the immunogen for the PSMB9 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PSMB9 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PSMB9 antibody is available for research use only.

Storage Conditions

Aliquot the PSMB9 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PSMB9 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human prostate carcinoma tissue with PSMB9 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.