Bcl-2-like protein 11 Antibody / BCL2L11 / Bim

Bcl-2-like protein 11, also called BCL2L11 and Bim, belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a Bcl-2 homology domain 3 (BH3) . It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X (L), and MCL1, and to act as an apoptotic activator.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:25, Flow cytometry: 1:50-1:100 (1x10e6 cells), Western blot: 1:500-1:1000

UniProt

O43521

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 134-160 from the human protein was used as the immunogen for the Bcl-2-like protein 11 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, FACS, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Bcl-2-like protein 11 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Bcl-2-like protein 11 antibody is available for research use only.

Storage Conditions

Aliquot the Bcl-2-like protein 11 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Bcl-2-like protein 11 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human colon carcinoma tissue with Bcl-2-like protein 11 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.