Dual specificity protein phosphatase 10 Antibody / DUSP10

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP10 binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. The protein is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q9Y6W6

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the Dual specificity protein phosphatase 10 antibody.

Clonality

Polyclonal

Isotype

Applications

IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Dual specificity protein phosphatase 10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Dual specificity protein phosphatase 10 antibody is available for research use only.

Storage Conditions

Aliquot the Dual specificity protein phosphatase 10 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Dual specificity protein phosphatase 10 antibody may be required due to differences in protocols and secondary/substrate sensitivity.