YWHAB Antibody / 14-3-3 beta/alpha

YWHAB belongs to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for the gene. Both variants encode the same protein, however, they are differentially expressed in hematopoietic cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P31946

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 217-246 from the human protein was used as the immunogen for the YWHAB antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the YWHAB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This YWHAB antibody is available for research use only.

Storage Conditions

Aliquot the YWHAB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the YWHAB antibody may be required due to differences in protocols and secondary/substrate sensitivity.