PFKM Antibody / Fructose 6 Phosphate Kinase

Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P) . Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P08237

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 746-776 from the human protein was used as the immunogen for the PFKM antibody.

Clonality

Polyclonal

Isotype

Applications

IF, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PFKM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PFKM antibody is available for research use only.

Storage Conditions

Aliquot the PFKM antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PFKM antibody may be required due to differences in protocols and secondary/substrate sensitivity.