PEPCK2 Antibody / PCK2

This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q16822

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 24-54 from the human protein was used as the immunogen for the PEPCK2 antibody.

Clonality

Polyclonal

Isotype

Applications

IF, WB, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the PEPCK2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PEPCK2 antibody is available for research use only.

Storage Conditions

Aliquot the PEPCK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the PEPCK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.