Steroid 21-hydroxylase Antibody / CYP21A2

CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100, Flow cytometry: 1:10-1:50 (1x10e6 cells)

UniProt

P08686

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 216-246 from the human protein was used as the immunogen for the Steroid 21-hydroxylase antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC-P, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Steroid 21-hydroxylase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Steroid 21-hydroxylase antibody is available for research use only.

Storage Conditions

Aliquot the Steroid 21-hydroxylase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Steroid 21-hydroxylase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human breast carcinoma tissue with Steroid 21-hydroxylase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.