Protein-glutamine gamma-glutamyltransferase 2 Antibody / Transglutaminase 2

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50

UniProt

P21980

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 429-458 from the human protein was used as the immunogen for the Protein-glutamine gamma-glutamyltransferase 2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Protein-glutamine gamma-glutamyltransferase 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Protein-glutamine gamma-glutamyltransferase 2 antibody is available for research use only.

Storage Conditions

Aliquot the Protein-glutamine gamma-glutamyltransferase 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Protein-glutamine gamma-glutamyltransferase 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic and cell surface

Image Legend

Western blot testing of human 1) HUVEC and 2) K562 cell lysate with Protein-glutamine gamma-glutamyltransferase 2 antibody. Predicted molecular weight ~78 kDa.