RAF1 Antibody / c-Raf
RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000
UniProt
P04049
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for the RAF1 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IF, IHC-P, WB
Purity
Antigen affinity purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the RAF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This RAF1 antibody is available for research use only.
Storage Conditions
Aliquot the RAF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Western blot testing of human Jurkat cell lysate with RAF1 antibody. Predicted molecular weight ~73 kDa.
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