RAF1 Antibody / c-Raf

RAF1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, RAF1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P04049

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the RAF1 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the RAF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This RAF1 antibody is available for research use only.

Storage Conditions

Aliquot the RAF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the RAF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.