Insulin receptor substrate 2 Antibody / IRS2

Insulin receptor substrate 2, a cytoplasmic signaling molecule that mediates effects of insulin, insulin-like growth factor 1, and other cytokines by acting as a molecular adaptor between diverse receptor tyrosine kinases and downstream effectors. This protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation, as well as by an interleukin 4 receptor-associated kinase in response to IL4 treatment.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q9Y4H2

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 1309-1338 from the human protein was used as the immunogen for the Insulin receptor substrate 2 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Insulin receptor substrate 2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Insulin receptor substrate 2 antibody is available for research use only.

Storage Conditions

Aliquot the Insulin receptor substrate 2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Insulin receptor substrate 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human HeLa cell lysate with Insulin receptor substrate 2 antibody. Predicted molecular weight ~137 kDa but it may be observed at up to ~190 kDa due to phosphorylation.