Beta-nerve growth factor Antibody / NGF

NGFB is a member of the NGF-beta family. It is a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in the gene encoding NGFB have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of the gene's expression is associated with allergic rhinitis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

P01138

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 84-115 from the human protein was used as the immunogen for the Beta-nerve growth factor antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Beta-nerve growth factor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Beta-nerve growth factor antibody is available for research use only.

Storage Conditions

Aliquot the Beta-nerve growth factor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Beta-nerve growth factor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, secreted

Image Legend

IHC testing of FFPE human brain tissue with Beta-nerve growth factor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.