FKBP1A Antibody / FKBP12

FKBP12 is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin. It interacts with several intracellular signal transduction proteins including type I TGF-beta receptor. It also interacts with multiple intracellular calcium release channels, and coordinates multi-protein complex formation of the tetrameric skeletal muscle ryanodine receptor. In mouse, deletion of this homologous gene causes congenital heart disorder known as noncompaction of left ventricular myocardium.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

P62942

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 55-83 from the human protein was used as the immunogen for the FKBP1A antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the FKBP1A antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This FKBP1A antibody is available for research use only.

Storage Conditions

Aliquot the FKBP1A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the FKBP1A antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human hepatocellular carcinoma tissue with FKBP1A antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.