MAPK3 Antibody / ERK1

ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2) . ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

P27361

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the MAPK3 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the MAPK3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This MAPK3 antibody is available for research use only.

Storage Conditions

Aliquot the MAPK3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MAPK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.