MAPK3 Antibody / ERK1
ERK1, a member of the MAP kinase subfamily of Ser/Thr protein kinases, is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. It phosphorylates EIF4EBP1 and is required for initiation of translation. The protein also phosphorylates microtubule-associated protein-2 (MAP2) . ERK1 is activated and tyrosine phosphorylated in response to insulin and NGF.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000
UniProt
P27361
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
A portion of amino acids 1-30 from the human protein was used as the immunogen for the MAPK3 antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
FACS, IHC-P, WB
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the MAPK3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MAPK3 antibody is available for research use only.
Storage Conditions
Aliquot the MAPK3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
Flow cytometry testing of human HeLa cells with MAPK3 antibody; Blue=isotype control, Green= MAPK3 antibody.
Frequently Asked Questions
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