GCAT Antibody / Glycine acetyltransferase

The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

O75600

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 155-181 from the human protein was used as the immunogen for the GCAT antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GCAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GCAT antibody is available for research use only.

Storage Conditions

Aliquot the GCAT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GCAT antibody may be required due to differences in protocols and secondary/substrate sensitivity.