GCAT Antibody / Glycine acetyltransferase
The degradation of L-threonine to glycine consists of a two-step biochemical pathway involving the enzymes L-threonine dehydrogenase and 2-amino-3-ketobutyrate coenzyme A ligase. L-Threonine is first converted into 2-amino-3-ketobutyrate by L-threonine dehydrogenase. GCAT is the second enzyme in this pathway, which then catalyzes the reaction between 2-amino-3-ketobutyrate and coenzyme A to form glycine and acetyl-CoA. The enzyme is considered a class II pyridoxal-phosphate-dependent aminotransferase.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100
UniProt
O75600
Host
Rabbit
Reactivity
Human, Mouse
Immunogen
A portion of amino acids 155-181 from the human protein was used as the immunogen for the GCAT antibody.
Clonality
Polyclonal
Isotype
Ig
Applications
WB, FACS, IHC-P
Purity
Purified
Format
Purified
Buffer
In 1X PBS, pH 7.4, with 0.09% sodium azide
Reconstitution
Aliquot the GCAT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This GCAT antibody is available for research use only.
Storage Conditions
Aliquot the GCAT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
In 1X PBS, pH 7.4, with 0.09% sodium azide
Applications Notes
Location
Cytoplasmic, nuclear
Image Legend
IHC testing of FFPE human brain tissue with GCAT antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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