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Pericentriolar material 1 protein Antibody / PCM1

PCM-1 is required for centrosome assembly and function. This protein is essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. The protein is required to anchor microtubules to the centrosome.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q15154

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 632-661 from the human protein was used as the immunogen for the Pericentriolar material 1 protein antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

IF, FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Pericentriolar material 1 protein antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pericentriolar material 1 protein antibody is available for research use only.

Storage Conditions

Aliquot the Pericentriolar material 1 protein antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Pericentriolar material 1 protein antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, centrosome

Image Legend

Western blot testing of human Ramos cell lysate with Pericentriolar material 1 protein antibody. Predicted molecular weight ~229 kDa.

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