Vitronectin Antibody / VTN

Vitronectin is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:1000-1:2000, Flow cytometry: 1:25 (1x10e6 cells)

UniProt

P04004

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 65-93 from the human protein was used as the immunogen for the Vitronectin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Vitronectin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Vitronectin antibody is available for research use only.

Storage Conditions

Aliquot the Vitronectin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Vitronectin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membrane

Image Legend

IHC testing of FFPE human lung carcinoma tissue with Vitronectin antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.