VCL Antibody / Vinculin

VCL/Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:10-1:50

UniProt

P18206

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A portion of amino acids 12-39 from the human protein was used as the immunogen for the VCL antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the VCL antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This VCL antibody is available for research use only.

Storage Conditions

Aliquot the VCL antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the VCL antibody may be required due to differences in protocols and secondary/substrate sensitivity.