GIT1 Antibody / p95-APP1

GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes (from SwissProt) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:50-1:100 (1x10e6 cells)

UniProt

Q9Y2X7

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 533-561 from the human protein was used as the immunogen for the GIT1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the GIT1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GIT1 antibody is available for research use only.

Storage Conditions

Aliquot the GIT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the GIT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.