Krueppel-like factor 5 Antibody / KLF5

KLF5 is a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, the protein is thought to be a transcription factor.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50

UniProt

Q13887

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 341-370 from the human protein was used as the immunogen for the Krueppel-like factor 5 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IF, FACS, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Krueppel-like factor 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Krueppel-like factor 5 antibody is available for research use only.

Storage Conditions

Aliquot the Krueppel-like factor 5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Krueppel-like factor 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Nuclear

Image Legend

IHC testing of FFPE human testis tissue with Krueppel-like factor 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.