Microfibrillar-associated protein 5 Antibody / MFAP5

MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2) . The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q13361

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 133-160 from the human protein was used as the immunogen for the Microfibrillar-associated protein 5 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Microfibrillar-associated protein 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Microfibrillar-associated protein 5 antibody is available for research use only.

Storage Conditions

Aliquot the Microfibrillar-associated protein 5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Microfibrillar-associated protein 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human skeletal muscle tissue with Microfibrillar-associated protein 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.