NADH-ubiquinone oxidoreductase chain 5 Antibody / MT-ND5

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:100

UniProt

P03915

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 544-570 from the human protein was used as the immunogen for the NADH-ubiquinone oxidoreductase chain 5 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, FACS, IHC-P

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the NADH-ubiquinone oxidoreductase chain 5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This NADH-ubiquinone oxidoreductase chain 5 antibody is available for research use only.

Storage Conditions

Aliquot the NADH-ubiquinone oxidoreductase chain 5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the NADH-ubiquinone oxidoreductase chain 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human liver tissue with NADH-ubiquinone oxidoreductase chain 5 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.