UDP-glucose 4-epimerase Antibody / GALE

UDP-galactose-4-epimerase catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q14376

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 142-171 from the human protein was used as the immunogen for the UDP-glucose 4-epimerase antibody.

Clonality

Polyclonal

Isotype

Applications

IF, FACS, IHC-P, WB

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the UDP-glucose 4-epimerase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This UDP-glucose 4-epimerase antibody is available for research use only.

Storage Conditions

Aliquot the UDP-glucose 4-epimerase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the UDP-glucose 4-epimerase antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin tissue with UDP-glucose 4-epimerase antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.