HLA-DRB1 Antibody

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages) . The beta chain is approximately 26-28 kDa. It is encoded by 6 exons.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)

UniProt

P04229

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 30-58 from the human protein was used as the immunogen for the HLA-DRB1 antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, FACS

Purity

Antigen affinity purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the HLA-DRB1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This HLA-DRB1 antibody is available for research use only.

Storage Conditions

Aliquot the HLA-DRB1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the HLA-DRB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.