SRSF1 Antibody / SF2

SRSF1 is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:50-1:100

UniProt

Q07955

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 158-186 from the human protein was used as the immunogen for the SRSF1 antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, WB, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SRSF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SRSF1 antibody is available for research use only.

Storage Conditions

Aliquot the SRSF1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SRSF1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human colon carcinoma tissue with SRSF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.