SMAC/Diablo Antibody

SMAC is an inhibitor of apoptosis protein (IAP) -binding protein. This mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:50-1:100, Western blot: 1:500-1:1000

UniProt

Q9NR28

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 211-239 from the human protein was used as the immunogen for the SMAC/Diablo antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the SMAC/Diablo antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This SMAC/Diablo antibody is available for research use only.

Storage Conditions

Aliquot the SMAC/Diablo antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the SMAC/Diablo antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human testis tissue with SMAC/Diablo antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.