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Calponin 1 Antibody

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunohistochemistry (FFPE) : 1:10-1:50

UniProt

P51911

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 10-39 from the human protein was used as the immunogen for the Calponin 1 antibody.

Clonality

Polyclonal

Isotype

Ig

Applications

WB, FACS, IHC-P

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Calponin 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Calponin 1 antibody is available for research use only.

Storage Conditions

Aliquot the Calponin 1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Calponin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Flow cytometry testing of human NCI-H292 cells with Calponin 1 antibody; Blue=isotype control, Green= Calponin 1 antibody.

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