Endothelin B Receptor Antibody / EDNRB

EDNRB is a G protein-coupled receptor which activates a phosphatidylinositol-calcium second messenger system. Its ligand, endothelin, consists of a family of three potent vasoactive peptides: ET1, ET2, and ET3. Studies suggest that the multigenic disorder, Hirschsprung disease type 2, is due to mutations in the endothelin receptor type B gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1:10-1:50 (1x10e6 cells), Immunofluorescence: 1:10-1:50, Immunohistochemistry (FFPE) : 1:10-1:50, Western blot: 1:500-1:1000

UniProt

P24530

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A portion of amino acids 409-439 from the human protein was used as the immunogen for the Endothelin B Receptor antibody.

Clonality

Polyclonal

Isotype

Applications

FACS, IF, IHC-P, WB

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the Endothelin B Receptor antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Endothelin B Receptor antibody is available for research use only.

Storage Conditions

Aliquot the Endothelin B Receptor antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the Endothelin B Receptor antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Image Legend

IHC testing of FFPE human prostate carcinoma tissue with Endothelin B Receptor antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.