M-CSF Antibody / Macrophage Colony Stimulating Factor 1 / CSF1

M-CSF is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. This protein may be involved in development of the placenta.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1:500-1:1000, Immunohistochemistry (FFPE) : 1:10-1:50, Immunofluorescence: 1:10-1:50, Flow cytometry: 1:10-1:50 (1x10e6 cells)

UniProt

P09603

Host

Rabbit

Reactivity

Human

Immunogen

A portion of amino acids 230-257 from the human protein was used as the immunogen for the M-CSF antibody.

Clonality

Polyclonal

Isotype

Applications

WB, IHC-P, IF, FACS

Purity

Purified

Format

Purified

Buffer

In 1X PBS, pH 7.4, with 0.09% sodium azide

Reconstitution

Aliquot the M-CSF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This M-CSF antibody is available for research use only.

Storage Conditions

Aliquot the M-CSF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

In 1X PBS, pH 7.4, with 0.09% sodium azide

Applications Notes

The stated application concentrations are suggested starting points. Titration of the M-CSF antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, membrane

Image Legend

IHC testing of FFPE human kidney tissue with M-CSF antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.